Open AccessOrthophosphate Turnover in the Extracellular and Intracellular Space of Mouse Liver
Author(s) -
Till U.,
Brox D.,
Frunder H.
Publication year - 1969
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1969.tb00806.x
Subject(s) - intracellular , extracellular , pi , flux (metallurgy) , chemistry , extracellular fluid , metabolism , biochemistry , organic chemistry
The flux rates of P i from blood plasma into the extracellular and intracellular space of mouse liver have been measured in vivo with the [ 32 P]P i ‐tracer technique. The specific radioactivities of plasma P i and total liver P i have been measured 0.1–4 min after injection of carrier‐free [ 32 P]P i . The results have been treated with the analogue computer technique. The main conclusions are:1 The flux rate of P i from blood plasma into the extracellular liver space amounts to 1.14 μmoles and that from the extracellular into the intracellular liver space to 0.39 μmoles per g fresh liver per min. 2 The influx of P i through the liver cell membrane takes place without transient transformation into another phosphate compound rapidly exchangeable with large intracellular phosphate pools. 3 The specific radioactivity of intracellular P i is somewhat higher than that of [γ‐ 32 P]ATP, thus indicating the absence of intracellular P i ‐compartmentation with isotope kinetic relevance.