Open AccessAffinity Chromatography of Bovine Pancratic Ribonuclease A
Author(s) -
Wilchek Meir,
Gorecki M.
Publication year - 1969
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1969.tb00799.x
Subject(s) - cyanogen bromide , ribonuclease , rnase p , affinity chromatography , chemistry , chromatography , conjugate , sepharose , cyanogen , uridine , bovine pancreatic ribonuclease , column chromatography , adsorption , enzyme , biochemistry , rna , organic chemistry , peptide sequence , mathematical analysis , mathematics , gene
5′‐(4‐Aminophenyl‐phosphoryl)‐uridine‐2′(3′)‐phosphate was synthesized and coupled to Sepharose by activation with cyanogen bromide. This conjugate was used as a specific adsorbent for purification of ribonuclease A by affinity chromatography. Totally reduced and oxidized RNase preparations which are enzymatically inactive were not adsorbed or retarded by the RNase‐specific column, while S‐protein is strongly retarded.