Open AccessSeparation of Two Types of Light Polypeptide Chains from Rabbit γ‐Globulins
Author(s) -
Vries G. M.,
Lanckman M.,
Hamers R.
Publication year - 1969
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1969.tb00782.x
Subject(s) - cystine , gamma globulin , size exclusion chromatography , chemistry , immunoglobulin light chain , population , amino acid , antibody , microbiology and biotechnology , biochemistry , biology , genetics , enzyme , medicine , environmental health , cysteine
The L chains from rabbit γ‐globulins can be separated by gel filtration into two populations (L1 and L2) differing markedly in their structural and antigenic proporties. The L1 and L2 chains from pooled IgG have different amino‐acid composition and half‐cystine content. In addition, each population can be further resolved in two subfractions called L1a and L1b, L2a and L2b, respectively. The b allotypic markers are located exclusively in L2 chains. These results indicate that the L chains from rabbit IgG are constituted by two populations of different genetic origin.