Open AccessThe Influence of Ribosomal Factors during the Maturation of Reticulocytes
Author(s) -
Herzberg M.,
Da D.,
Revel M.
Publication year - 1969
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1969.tb00752.x
Subject(s) - ribosome , polysome , ribosomal rna , protein biosynthesis , ribosomal protein , stimulation , microbiology and biotechnology , chemistry , biology , biochemistry , rna , gene , endocrinology
During maturation of reticulocytes their ribosomes become less active for protein synthesis. Testing the stimulation effect of KCl ribosomal wash (KCl factors) extracted from ribosomes from cells of different ages isolated by “density distribution of cells” technique, we show that active KCl factors are present on ribosomes from young cells and not on ribosomes from old cells. We demonstrate that the KCl factors can form polysomes from monosomes. The level of action of these factors is investigated and the possibility that they act as initiation factors is examined.