Open AccessHydrolysis of Vitamin A Acetate by Unspecific Carboxylesterases from Liver and Kidney
Author(s) -
Bertram J.,
Krisch K.
Publication year - 1969
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1969.tb00748.x
Subject(s) - hydrolysis , esterase , vitamin , pig liver , biochemistry , chemistry , kidney , fatty acid , chromatography , biology , enzyme , endocrinology
Vitamin A acetate is hydrolysed by highly purified carboxylesterases (EC 3.1.1.1) from liver and kidney of several species (pig, ox, man), whereas long‐chain fatty acid esters of vitamin A are not split. The hydrolysis of vitamin A acetate by pig liver esterase was characterized further by means of pH optimum, K m , specific and molecular activities and the behavior towards several inhibitors.