Open AccessThe Effect of Diethylether upon the Function of the Vesicles of Sarcoplasmic Reticulum
Author(s) -
Fiehn W.,
Hasselbach W.
Publication year - 1969
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1969.tb00647.x
Subject(s) - vesicle , calcium , ether , chemistry , endoplasmic reticulum , permeability (electromagnetism) , atpase , aqueous solution , calcium atpase , membrane , biophysics , membrane permeability , magnesium , biochemistry , chromatography , organic chemistry , enzyme , biology
1 The calcium permeability of the sarcoplasmic vesicles is greatly increased when vesicular suspensions are extracted with ether. 2 A 7–8% v/v aqueous ether solution abolishes the calcium concentrating ability of the vesicles completely. The calcium remaining in the solution permanently activates the calcium magnesium dependent ATPase. 3 In parallel to the change of the permeability of the vesicular membranes membranal lipids are removed by the ether treatment. Concentrations of ether which produce a maximal permeability increase only extract cholesterol esters. Higher concentrations of ether which inhibit the transport ATPase remove neutral lipids and phospholipids. The basic ATPase is not affected.