Open AccessTranslocation of some Anions Cations and Acids in Rat Liver Mitochondria
Author(s) -
Mitchell P.,
Moyle J.
Publication year - 1969
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1969.tb00588.x
Subject(s) - chromosomal translocation , antiporter , chemistry , choline , alkali metal , ion , phosphate , phase (matter) , inorganic chemistry , membrane , medicinal chemistry , biochemistry , organic chemistry , gene
Studies of net translocation of various anions and cations and their corresponding acids and bases, using extensions of established techniques, imply that the M phase of the cristae membrane of rat liver mitochondria is very impermeable ( i.e. not permitting net permeation) to the following ions: CH 3 COO − , H 2 PO 4 − , HPO 4 2− , SO 4 2− , Fe(CN) 6 3− , Fe(CN) 6 4− , choline + , K + , Na + and NH 4 + . How‐ever, the M phase appears to be permeated rapidly by SCN − and slowly by Cl − . It has been confirmed that CH 3 COO − and NH 4 + probably permeate as CH 3 COOH and NH 3 respectively, and that phosphate equilibrates across the M phase by an electrically neutral mechanism equivalent to H 3 PO 4 translocation. The possibility that phosphate is obligatorily translocated as KH 2 PO 4 has been eliminated. Sulphate equilibrates by an electrically neutral mechanism equivalent to H 2 SO 4 translocation. The alkali metals Na + and K + (and possibly NH 4 + ) equilibrate across the M phase via specific electrically neutral H + /Na + and H + /K + antiport, the former being the more active at neutral pH. Choline does not participate significantly in proton‐coupled antiport.