Open AccessReaction of Amino Acid‐tRNA Synthetases with 2′‐Deoxyadenosine Triphosphate
Author(s) -
Mitra S. K.,
Mehler A. H.
Publication year - 1969
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1969.tb00578.x
Subject(s) - transfer rna , pyrophosphate , nucleotide , enzyme , adenosine triphosphate , biochemistry , chemistry , deoxyadenosine , amino acid , rna , stereochemistry , gene
All of the amino acid activating enzymes studied are capable of reacting with dATP in place of ATP. None of the other available nucleotides was found to be used by these enzymes. The K m of dATP is generally higher than that of ATP but the maximum velocities are similar in the complete reaction, esterification of transfer RNA. The rates of pyrophosphate exchange are much lower with dATP than with ATP in the cases studied.