Open AccessThe Nucleotide Sequence of N ‐Formyl‐Methionyl‐Transfer RNA
Author(s) -
Dube S. K.,
Marcker K. A.,
Clark B. F. C.,
Cory S.
Publication year - 1969
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1969.tb00521.x
Subject(s) - nucleotide , ribonuclease , ribonuclease t1 , pancreatic ribonuclease , rna , s tag , ribonuclease iii , chemistry , sephadex , biochemistry , bovine pancreatic ribonuclease , digestion (alchemy) , column chromatography , chromatography , enzyme , rnase p , rna interference , gene
32 P‐labelled N ‐formyl methionyl transfer RNA from Escherichia coli strain CA265 was purified by chromatography on a column of DEAE Sephadex followed by chromatography on benzoylated DEAE cellulose. Nucleotides from ribonuclease T 1 and pancreatic ribonuclease digests of tRNA F were separated and their sequences determined. tRNA F consists of 77 nucleotides of which pCG is 5′‐terminal and CAACCA OH is 3′‐terminal.