Open AccessAmino Acid Control of RNA Synthesis in T4‐Infected Escherichia coli
Author(s) -
Olsnes S.,
Hauge J. G.
Publication year - 1968
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1968.tb19583.x
Subject(s) - uracil , escherichia coli , rna , polysome , protein biosynthesis , amino acid , messenger rna , strain (injury) , stringent response , biology , biochemistry , chemistry , ribosome , gene , dna , anatomy
A comparison has been made of incorporation of [5‐ 3 H]uracil after T4‐infection in several strains of Escherichia coli that show stringent control of RNA synthesis in the uninfected state. Some of the strains exhibited a greatly reduced initial rate of [ 3 H]uracil incorporation when a required amino acid was removed. The nature of the reduced [ 3 H]uracil incorporation was investigated for one strain and shown by hybridization competition to reflect a reduced rate of T4 mRNA synthesis. In other strains, [ 3 H]uracil incorporation was not appreciably affected by amino acid starvation. RNA synthesized under these conditions entered polysomes at a high rate in spite of a low rate of protein synthesis.