Open AccessBiosynthesis of Two New Steroids in the Human Foetal Liver, 1 β‐ and 2β‐Hydroxytestosterone
Author(s) -
Lisboa B. P.,
Gustafsson J.Å.
Publication year - 1968
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1968.tb00463.x
Subject(s) - biosynthesis , microsome , incubation , chemistry , chromatography , human liver , mass spectrometry , biochemistry , enzyme
Two new C 19 ‐steroids with a 3‐oxo‐Δ 4 ‐structure have been isolated and identified after incubation of testosterone with 105,000 × g microsomes of human foetal liver. These two ultraviolet positive metabolites were isolated by ascending one‐dimensional thin‐layer chromatography. The isolated compounds were identified as 1β‐ and 2β‐hydroxytestosterone by means of gas chromatography‐mass spectrometry. They were formed in approximately the same amount, each one representing about 15 % of the amount of 6β‐hydroxytestosterone formed.