Open AccessProteine aus Neurospora crassa
Author(s) -
Friemel H.,
Peseke H.,
Kleist H.,
Brock J.,
Bienengräber V.,
Mücke D.
Publication year - 1968
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1968.tb00455.x
Subject(s) - neurospora crassa , crassa , antiserum , mycelium , biology , immunoelectrophoresis , electrophoresis , chromatography , agar plate , agar , biochemistry , polyacrylamide gel electrophoresis , chemistry , mutant , enzyme , botany , antigen , genetics , gene , bacteria
The mycelium of Neurospora crassa was cultivated in a minimal liquid medium in surface culture. The moist mycelium was lyophilized, powdered, and eluted using 0,14 M sodium chloride solution for extracting the proteins. The enrichment of these proteins was carried out by means of ultrafiltration (vacuum and pressure filtration). The extracted proteins of N. crassa were characterized by paper‐, agar gel‐ and immuno‐electrophoresis. The antisera were obtained by immunising rabbits for 21 weeks. By means of agar gel electrophoresis 5 zones of proteins were separated in N. crassa 3 a 6A; by means of immunoelectrophoresis 17 bands resulted from the same protein mixture. The electrophoretic mobilities of the precipitation arcs were calculated. The immunoelectrophoretic patterns of N. crassa E 5256 obtained by N. crassa 3 a 6A antiserum nearly agreed with the bands of N. crassa 3 a 6A antigen. However there were no corresponding results in the patterns obtained from N. crassa 46001–1–1a and N. crassa 6994a with the same antiserum. It may be assumed that these differences depend on the different genetic enzyme patterns of the wild and mutant strains; they are also responsible for the differences in the rates of synthesis depending on the duration of cultivation.