Open AccessSerine Specific Transfer Ribonucleic Acids
Author(s) -
Zachau H. G.
Publication year - 1968
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1968.tb00406.x
Subject(s) - chemistry , dissociation (chemistry) , serine , monomer , elution , transfer rna , sephadex , phenol , chromatography , enzyme , biochemistry , rna , organic chemistry , polymer , gene
Aggregates of two and more tRNA Ser molecules are formed from purified tRNA Ser yeast under commonly used storage conditions. The aggregates can also be produced easily by adjusting to pH 3.0 a solution of tRNA Ser , Ser‐tRNA Ser , or unfractionated tRNA and subsequently adding a neutral Mg ++ ‐containing buffer. The various aggregates were characterized by Sephadex elution profiles and by the rates of dissociation at elevated temperatures. At a given temperature, EDTA and phenol increase the rate of dissociation. The tRNA Ser aggregates were found to accept serine under the conditions of the enzymatic charging of the tRNA Ser monomer. The rate of charging is lower and the level of charging is only 1/3 to 1/2 of the one, which can be reached with the same aggregate sample after dissociation prior to the assay. Since the control experiments with the aim to explain the serine incorporation as an artefact (enzyme bound radioactivity; aggregate charging via charging of monomeric tRNA) were negative, the idea of a direct charging of the aggregates was accepted.