Open AccessChemical Studies on the Specific Fragment of Shigella sonnei Phase II
Author(s) -
Romanowska E.,
Mulczyk M.
Publication year - 1968
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1968.tb00343.x
Subject(s) - shigella sonnei , isoelectric point , chemistry , galactose , chromatography , isoelectric focusing , hydrolysis , glucosamine , sugar , heptose , size exclusion chromatography , biochemistry , acid hydrolysis , galactosamine , microbiology and biotechnology , shigella , biology , escherichia coli , enzyme , mutant , gene
The pure lipopolysaccharide of Shigella sonnei phase II was submitted to acid hydrolysis (0.1 N H 2 SO 4 , 20 min, 100°), and from the hydrolyzate the specific fragment was isolated and purified. The analysis of the specific fragment showed that it contained about 70% sugar; the major constituents were glucose, galactose and heptose in the approximate proportion 3:2:2.2. 2‐Keto‐3‐deoxyoctonate (3.2%), glucosamine (3.4%) and O ‐phosphorylethanolamine (6.0%) were also found. The molecular weight of the specific fragment estimated by gel filtration ranged between 4,500–5,000. Electrophoretic experiments indicated its acid character: the isoelectric point was near 2.9.