Open AccessInfrared Studies of Deoxyribonucleic Acids, their Constituents and Analogues
Author(s) -
Fritzsche H.,
Zimmer C.
Publication year - 1968
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1968.tb00334.x
Subject(s) - guanine , copper , cytosine , chemistry , infrared , dna , infrared spectroscopy , crystallography , nucleic acid , spectral line , nucleobase , nucleotide , stereochemistry , biochemistry , organic chemistry , physics , astronomy , optics , gene
The infrared spectra of the four deoxynucleosides dT, dA, dG, and dC have been studied in 2 H 2 O solution without and with addition of CuSO 4 . The infrared spectra of dT and dA remain unchanged by addition of copper(II), whereas new bands appear at 1,517 and 1,547 cm −1 in the infrared spectrum of dC and at 1,590 cm −1 in the infrared spectrum of dG after addition of CuSO 4 . The changes in the infrared spectra of DNA from Streptomyces chrysomallus (72 mole% guanine + cytosine) after interaction with copper(II) are very similar to the observed changes in the nucleoside spectra New bands at 1,518 and 1,551 cm −1 indicate the interaction of cytosine bases of DNA with copper(II), the band at 1,590 cm −1 indicates the interaction of guanine bases of DNA with copper(II).