Open AccessEnergy Requirements in the Incorporation of Choline by Isolated Rat Liver Mitochondria
Author(s) -
BYGRAVE F. L.,
KAISER W.
Publication year - 1968
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1968.tb00252.x
Subject(s) - oxidative phosphorylation , choline , mitochondrion , phospholipid , biochemistry , nucleotide , chemistry , atp synthase , phosphorylation , electron transport chain , respiration , metabolism , energy metabolism , adenosine triphosphate , nad+ kinase , biology , membrane , enzyme , endocrinology , gene , anatomy
Optimal concentrations of ATP, CTP and ADP for the incorporation of 14 C‐labelled choline into phospholipid by isolated rat liver mitochondria were 0.7 mM, 0.25 mM and 1.0 mM respectively. Higher concentrations markedly depressed the incorporation. Substitution of CTP for other nucleotide triphosphates led to lower rates of incorporation. Inhibitors of electron transport and of oxidative phosphorylation inhibited the respiration‐supported but not the ATP‐supported incorporation of choline. It is concluded that high energy intermediates of oxidative phosphorylation are not obligatory for the incorporation process.