Open AccessThe Fractionation of the Histones of Group F2(b) from Calf Thymus
Author(s) -
JOHNS E. W.
Publication year - 1968
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1968.tb00231.x
Subject(s) - acetone , fractionation , chromatography , chemistry , precipitation , column chromatography , proline , extraction (chemistry) , amino acid , organic chemistry , biochemistry , physics , meteorology
The histones of group F2(b) have been fractionated by acetone precipitation and by column chromatography on carboxymethylcellulose at pH 9. The acetone precipitation method enables F2(b), prepared on a large scale by extraction methods, to be further purified in bulk, the resulting product having 84% N‐terminal proline. The column chromatography on carboxymethylcellulose at pH 9 has given 5 main fractions, two of which contain over 25% acidic amino acids (or their amides) and over 24% basic amino acids.