Open AccessFormation of Polysomes in T2‐Infected Escherichia coli during Inhibition of Protein Synthesis
Author(s) -
HAUGE J. G.
Publication year - 1968
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1968.tb00230.x
Subject(s) - polysome , rna , methionine , escherichia coli , protein biosynthesis , uracil , centrifugation , biochemistry , sucrose , amino acid , biology , dna , ribosome , chemistry , microbiology and biotechnology , gene
A comparison has been carried out between RNA made in the presence and absence of methionine in a T2‐infected RC stringent Escherichia coli requiring methionine. RNA was labelled with H 32 PO 4 ‐or [ 3 H]uracil for 4 minutes during the early phase of the infection. After sucrose density gradient centrifugation, the radioactive RNA formed had highest specific activity in the polysome region, and this activity was essentially the same for the two experimental conditions. The radioactive RNA from the polysomes was demonstrated to be hybridizable with T2 DNA. The findings indicate the presence of a mechanism which allows polysome formation and RNA synthesis to proceed normally under circumstances where deprivation of a required amino acid greatly reduces protein synthesis.