Open AccessReactions Sequence of Leucine Activation Catalysed by Leucyl‐RNA Synthetase
Author(s) -
ROUGET P.,
CHAPEVILLE F.
Publication year - 1968
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1968.tb00210.x
Subject(s) - leucine , enzyme , rna , biochemistry , chemistry , sequence (biology) , stereochemistry , amino acid , gene
In order to obtain information on the sequence of reactions which participate in the activation of leucine, the formation of complexes between the leucyl‐RNA synthetase and its substrates was examined. A leucyladenylate‐enzyme complex able to transfer its leucine to the RNA was isolated. Likewise, an enzyme‐ATP complex was separated; when re‐incubated with leucine, it reacts to form the leucyladenylate‐enzyme complex. The effect of leucinol, leucinol‐AMP and 6‐ N ‐hydroxyethyl‐ATP on the formation of these complexes was examined. The results indicate that during the activation of leucine, the leucyl‐RNA synthetase first binds ATP and then reacts with leucine.