Open AccessUptake of Amino Acids by Mucosa of Rat Colon in vitro
Author(s) -
EVERED D. F.,
NUNN P. B.
Publication year - 1968
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1968.tb00208.x
Subject(s) - valine , glycine , methionine , amino acid , intestinal mucosa , biochemistry , in vitro , histidine , chemistry , arginine , citrulline , biology , medicine
1 Rings of mucosa from rat colon accumulated l ‐[ 14 C 5 ]valine against a concentration gradient. The accumulation of l ‐valine was inhibited to some extent by dinitrophenol and cyanide, and more markedly by glycine and l ‐methionine. l ‐Lysine was ineffective as an inhibitor. 2 Using everted sacs of colonic mucosa, l ‐valine, l ‐citrulline and l ‐histidine permeated the mucosa from the mucosal fluid to the serosal fluid down a concentration gradient. In experiments with a single amino acid in both the serosal and the mucosal fluids there was always a net loss of amino acid from the serosal fluid. 3 These results are discussed in relation to both tissue accumulation and intestinal absorption of amino acids.