Open AccessEarly Effects of Phytohaemagglutinin on Lymphocyte RNA Synthesis
Author(s) -
KAY J. E.
Publication year - 1968
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1968.tb00198.x
Subject(s) - phytohaemagglutinin , rna , labelling , cytoplasm , incubation , microbiology and biotechnology , uridine , biology , biochemistry , ribosomal rna , in vitro , gene
1 Phytohaemagglutinin was found to cause a rapid increase in the incorporation of [ 3 H]‐uridine into lymphocyte RNA and an increase in the rate of incorporation of [ 14 C]leucine into protein which began about 2 h after its addition. 2 During the first few hours after its addition phytohaemagglutinin stimulated the labelling of cytoplasmic RNA more than the labelling of nuclear or whole cell RNA. 3 The cytoplasmic RNA labelled during the first 2 h of incubation of lymphocytes with phytohaemagglutinin sedimented mainly at 4 S when analyzed by sucrose gradient centrifugation, but part of it could be distinguished from transfer RNA. Phytohaemagglutinin stimulated the labelling of both fractions of the labelled cytoplasmic RNA more than the labelling of whole cell RNA. 4 Labelled ribosomal RNA was first found in the cytoplasm after incubation of lymphocytes with [ 3 H]uridine for 3 h. Incubation with phytohaemagglutinin markedly stimulated the labelling of this fraction of RNA.