Open AccessRibosomal Precursor RNA in Saccharomyces carlsbergensis
Author(s) -
Retèl J.,
Planta R. J.
Publication year - 1967
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1967.tb19524.x
Subject(s) - ribosomal rna , rna , 5.8s ribosomal rna , 5s ribosomal rna , transfer rna , ribosome , rna polymerase i , 18s ribosomal rna , yeast , biology , microbiology and biotechnology , messenger rna , biochemistry , chemistry , rna dependent rna polymerase , gene
Several lines of evidence for the occurrence of ribosomal precursor RNA in yeast are presented. This ribosomal precursor RNA has been found in the nuclear fraction, it is rapidly labeled with radioactive nucleotides and sediments faster than the two ribosomal RNAs. In addition another rapidly labeled RNA of high molecular weight has been found, which is turning over rapidly and is presumably of messenger nature. It proved to be possible to differentiate between both kinds of rapidly labeled RNA by using the technique of “shift‐up” and “shift‐down” in conjunction with pulse‐labeling.