Open AccessSynchronized Yeast Cells
Author(s) -
Eckstein H.,
Paduch V.,
Hilz H.
Publication year - 1967
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1967.tb19520.x
Subject(s) - dna polymerase , dna replication , dna , yeast , dna clamp , polymerase , biology , saccharomyces cerevisiae , biochemistry , primer (cosmetics) , dna polymerase ii , dna polymerase i , transcription (linguistics) , s phase , microbiology and biotechnology , dna synthesis , chemistry , gene , eukaryotic dna replication , polymerase chain reaction , reverse transcriptase , linguistics , philosophy , organic chemistry
Homogenates and extracts of yeast cells ( Saccharomyces cerevisiae ) exhibit DNA polymeras activity as revealed by incorporation of labeled dATP into DNAase‐sensitive, RNAase‐resistent acid‐insoluble material, the incorporation being dependent on the presence of all four deoxynucleotide triphosphates and primer DNA. Extracts from synchronized yeast cells exhibit DNA polymerase activity in an oscillating manner during the cell cycle, maximum activity being found just before the onset of DNA replication. Degradation and resynthsis, or transformation to an inactive “zymogen” are discussed as possible mechanisms of the oscillating behavior of polymerase activity. X‐irradiation brings about a dissociation of DNA replication and oscillating appearance of the polymerase. Whereas DNA replication is temporarily delayed, the rhythmic appearance of the enzyme is unaffected. However, the height of the activity maxima remains unchanged until DNA is reduplicated again. The observations are discussed with regard to the apparent intactness of the DNA template functions and to the persistence of the sequential transcription of genes in the proliferation‐inhibited irradiated yeast cells.