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Impact of solvent/detergent treatment of plasma on transfusion‐relevant bacteria
Author(s) -
Chou M.L.,
Wu Y.W.,
Su C.Y.,
Lee L.W.,
Burnouf T.
Publication year - 2012
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.2011.01560.x
Subject(s) - medicine , intensive care medicine , bacteria , blood transfusion , chemistry , immunology , biology , genetics
Background A solvent/detergent (S/D) treatment in a medical device has been developed for pathogen reduction of plasma for transfusion. Impact of S/D on bacterial growth and on the capacity of complement to kill bacteria has been investigated in this study. Study design and methods A pool of apheresis plasma from four donors was spiked with eight transfusion‐relevant bacteria. Plasma was treated with 1% tri(n‐butyl) phosphate and 1% Triton X‐45 at 31°C for 90 min and then extracted by oil at 31°C for 70 min. Decomplemented plasma and Phosphate Buffer Saline were used as controls. Bacterial count was determined in samples taken immediately after spiking, or after S/D and oil treatment. Similar experiments were conducted using three individual recovered plasma donations. Bacteria growth inhibition tests were performed using discs soaked with plasma samples whether containing the S/D agents or not. Results The mean reduction factors of Escherichia coli , Pseudomonas aeruginosa , and Klebsiella pneumoniae due to complement during S/D treatment were >8·75, 4·71, and 4·18 log in pooled plasma and >7·42, 2·24 and >6·08 log in individual plasmas, respectively. Bacillus cereus and Bacillus subtilis were inactivated by S/D (>7·04 and 1·60 log in pooled, and >6·06 and 2·39 in individual plasmas, respectively). Staphylococcus aureus , Staphylococcus epidermidis and Enterobacter cloacae did not multiply during S/D treatment of plasma. Growth inhibition tests revealed an inhibition of three Gram‐negative bacteria by complement and all Gram‐positive by S/D. Conclusion The S/D treatment of plasma does not alter the bactericidal activity of complement, and inactivates some Gram‐positive bacteria.