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The effect of plasma removal from apheresis platelet concentrates on platelet function
Author(s) -
JilmaStohlawetz P.,
Eichelberger B.,
List J.,
Jilma B.,
Panzer S.,
Leitner G.
Publication year - 2012
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.2011.01548.x
Subject(s) - platelet , centrifugation , apheresis , chemistry , chromatography , platelet rich plasma , plasma , immunology , medicine , physics , quantum mechanics
The effect of plasma removal on platelet function has scarcely been investigated. Plasma removal from apheresis platelet concentrates was achieved by centrifugation at 5000 g for 6 min or 2000 g for 10 min. After resting for 1 h, platelet concentrates were resuspended in 0·9% NaCl. Platelet function was tested before centrifugation and after resuspension by multiple electrode impedance aggregometry (MEA) and light transmission aggregometry (LTA). Plasma removal resulted in 10–14% lower response to TRAP‐6 by MEA using both washing procedures, whereas TRAP‐6‐inducible aggregation by LTA increased slightly (2–5%). Neither plasma removal method affected collagen‐induced aggregation. Thus, platelet function did not deteriorate significantly by either method.