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Feasibility of a closed‐system cell processor (ACP215) for automated preparation of washed platelet concentrates
Author(s) -
Oikawa S.,
Sasaki D.,
Kikuchi M.,
Sawamura Y.,
Itoh T.
Publication year - 2012
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.2011.01520.x
Subject(s) - platelet , chemistry , platelet rich plasma , platelet concentrate , chromatography , in vitro , platelet activation , biomedical engineering , medicine , biochemistry , immunology
Background and Objectives This study was aimed at evaluating the feasibility of the ACP215 closed‐system cell processor for preparing washed platelet concentrates. Material and Methods Platelet washing was performed with either the ACP215 system or the manual technique with M‐sol. Plasma protein removal and platelet recovery were estimated, and the washed platelet concentrates were stored for 5 days. Samples were collected after washing and on days 1, 3 and 5 of storage to determine the effects of the washing methods on the in vitro platelet qualities (platelet count, platelet volume, pH, glucose and lactate concentrations, hypotonic shock response, aggregation response and CD62P expression level). Results Platelet recovery was 86·9 ± 2·1% and 85·9 ± 1·9% ( P = 0·305), and plasma protein removal was 95·8 ± 0·9% and 96·9 ± 0·7% ( P = 0·016) after washing with the ACP215 system and manual technique, respectively. No statistically significant differences in the in vitro platelet qualities were observed between the washing methods. Conclusion The ACP215 system is a feasible alternative to manual, labour‐intensive, techniques for preparing washed platelet concentrates.