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Characteristics of prion‐filtered red cells suspended in pathogen‐inactivated plasma (MB treated or solvent‐detergent treated) for neonatal exchange transfusion
Author(s) -
Hornsey V. S.,
Casey C.,
McColl K.,
Young H.,
Drummond O.,
McMillan L.,
Morrison A.,
Prowse C. V.
Publication year - 2011
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.2010.01456.x
Subject(s) - haemolysis , red cell , chemistry , rose bengal , mannitol , fibrinogen , methylene blue , platelet , lysis , blood proteins , potassium , andrology , biochemistry , microbiology and biotechnology , biology , medicine , immunology , catalysis , organic chemistry , photocatalysis
Background and Objectives Neonates undergoing exchange transfusion require < 5‐day‐old red cells suspended in plasma. This study assesses the effect of replacing the saline, adenine, glucose and mannitol (SAGM) of prion reduced (P‐Capt) red cells with either methylene blue–treated plasma (MBTFFP) or OctaplasLG to reduce the risk of variant Creutzfelt–Jakob disease transmission. Materials and Methods Twenty leucoreduced red cell units in SAGM were prion reduced on day 1. The SAGM was replaced by MBTFFP ( n = 10) or OctaplasLG ( n = 10). The units were irradiated and stored at 4°C for 24 h. A further 20 units were stored for 5 days before being processed as above. Haemolysis (%), potassium, ATP, 2,3‐DPG and plasma proteins were measured. Results Haemolysis remained low (≤ 0·16%). Following irradiation and storage, red cells in both types of plasma showed similar changes in potassium and ATP concentrations. The 2,3‐DPG concentrations were well maintained although lower in red cells in OctaplasLG compared with those in MBTFFP (4·79 vs. 6·83 μmoles/g Hb on day 6). MBTFFP contained lower concentrations of fibrinogen, FV and FVIII. In OctaplasLG, alpha‐2‐antiplasmin was approximately 0·4 U/ml lower than in MBTFFP. After 24 h at 4°C, free protein S in OctaplasLG fell from 0·82 to 0·57 IU/ml. Other plasma proteins, in both types of plasma, were stable. Conclusions Red cells in both types of plasma demonstrated similar storage characteristics. The plasma proteins, except protein S in OctaplasLG, were stable over 24 h at 4°C in both types of plasma, and low FVIII concentrations were noted in the MBTFFP (group O) units used.