Premium
PEGylation prevents bacteria‐induced platelet activation and biofilm formation in platelet concentrates
Author(s) -
Greco C. A.,
MaurerSpurej E.,
Scott M. D.,
Kalab M.,
Nakane N.,
RamírezArcos S. M.
Publication year - 2011
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.2010.01419.x
Subject(s) - biofilm , staphylococcus epidermidis , pegylation , platelet , bacteria , chemistry , microbiology and biotechnology , contamination , platelet activation , staphylococcus aureus , biochemistry , polyethylene glycol , immunology , biology , ecology , genetics
Bacterial contamination of platelet concentrates represents the greatest post‐transfusion infectious risk. Biofilm formation in this environment resulting from platelet–bacteria interactions can lead to non‐uniform contaminant distribution and thus missed detection. As formation of platelet–bacteria aggregates is largely based on receptor–ligand interactions, we examined whether shielding these events would result in reduced biofilm formation by contaminant bacteria. We introduced methoxypoly(ethylene glycol) to covalently modify the platelet surface using a process termed ‘PEGylation’. In the first study of its kind, we demonstrate that PEGylated platelet concentrates inoculated with Staphylococcus epidermidis display a significant reduction in bacterial binding and biofilm formation.