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Analysis of Wnt pathway genes during ex vivo expansion and neutrophil differentiation of umbilical cord‐blood derived CD34 + cells
Author(s) -
Gallagher R. C. J.,
TuraCeide O.,
Turner M.,
Barclay R.
Publication year - 2010
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.2009.01299.x
Subject(s) - ex vivo , umbilical cord , cd34 , progenitor cell , cord blood , biology , wnt signaling pathway , progenitor , microbiology and biotechnology , stem cell , immunology , in vivo , genetics , signal transduction
Previous work has shown that optimal ex vivo expansion and differentiation of CD34 + progenitor cells into neutrophils is by addition of Flt3‐L, SCF and G‐CSF. Here we report that a variety of genes involved in the WNT pathway are transcriptionally active in both undifferentiated and differentiated umbilical cord blood CD34 + cells, however statistically significant changes in gene expression are not always consistent across UCB samples.