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Mirasol Pathogen Reduction Technology ® treatment does not affect acute lung injury in a two‐event in vivo model caused by stored blood components
Author(s) -
Silliman C. C.,
Khan S. Y.,
Bradley Ball J.,
Kelher M. R.,
Marschner S.
Publication year - 2010
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.2009.01289.x
Subject(s) - priming (agriculture) , in vivo , packed red blood cells , immunology , medicine , biology , blood transfusion , botany , germination , microbiology and biotechnology
Mirasol Pathogen Reduction Technology ® (PRT) treatment uses riboflavin and UV light to inactivate pathogens in blood components. Neutrophil [polymorphonuclear cells (PMN)] priming activity accumulates during routine storage of cellular blood components, and this activity has been implicated in transfusion‐related acute lung injury (TRALI). We hypothesize that PRT‐treatment of blood components affects the priming activity generated during storage of packed RBCs (PRBCs) or platelet concentrates (PCs), which can elicit ALI in vivo . Methods Plasma, PRBCs and PCs were isolated from healthy donor’s whole blood or by apheresis. Half of a collected unit was treated with PRT treatment and the remainder was left as an unmodified control. Supernatant was collected during storage of PCs and PRBCs and assayed for PMN priming activity and used as the second event in a two‐event in vivo model of TRALI. Results PRT treatment did not induce priming activity in plasma or affect the priming activity generated during storage of PCs or PRBCs as compared with the unmodified controls. The supernatants from stored, but not fresh, PCs and PRBCs did cause ALI as the second event in a two‐event animal model of TRALI, which was unaffected by PRT treatment. We conclude that the PRT ® treatment does not induce priming activity in plasma nor does it affect the priming activity generated during storage of PCs or PRBCs or their ability to cause ALI as the second event in a two‐event in vivo model of TRALI. Moreover, the amount of priming activity in TRIMA ® ‐isolated PCs was significantly less than SPECTRA ® ‐isolated PCs.