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Pathogen reduction technology (Mirasol ® ) treated single‐donor platelets resuspended in a mixture of autologous plasma and PAS
Author(s) -
Janetzko K.,
Hinz K.,
Marschner S.,
Goodrich R.,
Klüter H.
Publication year - 2009
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.2009.01193.x
Subject(s) - platelet , chemistry , metabolism , blood product , food science , biochemistry , chromatography , andrology , medicine , surgery
Background and Objectives Mirasol ® pathogen reduction technology (PRT) for platelet concentrates uses riboflavin and ultraviolet light. Previously, we described increased metabolism and activation for PRT platelets stored in 100% plasma. To improve platelet quality, we resuspended platelets in a mixture of plasma and platelet additive solution (PAS). Materials and Methods Single‐donor platelets were resuspended in plasma and split into an untreated control and a PRT‐treated single product. One hundred and fifty millilitre PAS (SSP+) was added to both. Over 7 days, we assayed pH, glucose consumption‐, lactate production rate and CD62p with and without TRAP. Results On day 5, PRT units showed a significantly lower pH (7·087 ± 0·105 vs. 7·288 ± 0·200) accompanied by a higher lactate production (0·104 ± 0014 vs. 0·063 ± 0·017 mmol/10 12 /h) and glucose consumption rate (0·039 ± 0005 vs. 0·028 ± 0·009 mmol/10 12 platelets/h). CD62p expression was higher in treated units (44·5 ± 13·0 vs. 16·5 ± 7·6%). Conclusion In comparison to PRT platelets resuspended in 100% plasma, a mixture of plasma and PAS improves pH and platelet metabolism but not platelet activation. Prolonged shelf‐life for up to 7 days may be possible.