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Improved leucoreduction of red blood cell units prepared after a 24‐h hold with the platelet‐rich plasma method using newly developed filters
Author(s) -
Thibault L.,
Beauséjour A.,
Jacques A.,
De Grandmont M. J.,
Lemieux R.,
Grégoire Y.,
Ducas É.,
Boucher G.
Publication year - 2008
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.2007.01032.x
Subject(s) - platelet , red blood cell , blood cell , red cell , chromatography , chemistry , medicine , immunology
Background and Objectives A previous study indicated that the extension of whole blood (WB) storage from 8 to 24 h at 20–24 °C before the processing of platelet‐rich plasma (PRP)‐depleted red blood cell (RBC) units had a negative effect on the efficacy of leucoreduction filters. In this study, we further characterized the phenomenon and tested the leucoreduction capacity of two newly developed filters. Materials and Methods Whole blood was stored at 20–24 °C and processed at 4‐h intervals between 8 and 24 h postcollection. Components were leucoreduced before storage. Efficacy of novel filters to leucoreduce 24‐h‐hold PRP‐depleted RBC units was also evaluated. Results Using a conventional filter, the mean residual white blood cell (WBC) counts in leucoreduced PRP‐depleted RBCs were comparable in units prepared within 12 h from collection but gradually increased upon extended preprocessing storage from 0·36 ± 0·03 at 12 h to 0·46 ± 0·21, 0·76 ± 0·54 and 1·72 ± 1·76 × 10 6 per unit at 16, 20 and 24 h, respectively. However, the mean residual WBC content in 24‐h‐hold RBCs was reduced to 0·60 ± 0·39 × 10 6 and 0·46 ± 0·13 × 10 6 per units using RC2D and the prototypes B‐1582 rev B filters, respectively. Conclusion For PRP‐depleted RBC units, the extension of the WB room temperature storage from 8 to 24 h before processing is likely to require the introduction of newly developed filters having an increased leucoreduction capacity in order to meet the maximal residual WBC guideline in the RBCs.