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The level of matrix metalloproteinase‐2 in donor plasma strongly correlates with post‐transfusion recovery of stored platelet concentrates
Author(s) -
Mutlu A.,
Allen D. J.,
Freedman J.,
Leytin V.
Publication year - 2008
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.2007.01001.x
Subject(s) - platelet , matrix (chemical analysis) , matrix metalloproteinase , medicine , matrix metalloproteinase 3 , platelet transfusion , chemistry , immunology , chromatography
Background and Objective A simple test accurately predicting post‐transfusion recovery of stored platelet concentrates (PCs) is needed. Materials and Methods Matrix metalloproteinase‐2 (MMP‐2) was determined in fresh‐frozen plasma of normal donors using enzyme‐linked immunosorbent assay (ELISA)‐based assay. Post‐transfusion recovery of human PCs was determined in rabbits with an inhibited reticuloendothelial system by flow cytometry. Results We demonstrated a strong positive ( r = 0·91) and highly significant ( P = 0·0015) correlation between the concentration of MMP‐2 in plasma of normal donors and post‐transfusion recovery of PCs stored for 5 days at 22 °C under standard blood banking conditions. A higher level of donor plasma MMP‐2 is associated with improved recovery of transfused platelets. Conclusion These observations suggest that pre‐transfusion MMP‐2 assay of donor's plasma may be a predictor of post‐transfusion recovery of PCs. However, the usefulness of this assay should be elucidated in clinical transfusion settings.