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Collection of hyperconcentrated platelets with Trima Accel
Author(s) -
Ringwald J.,
Duerler T.,
Frankow O.,
Zimmermann R.,
Zingsem J.,
Strasser E.,
Antoon M.,
Eckstein R.
Publication year - 2006
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.2005.00721.x
Subject(s) - apheresis , platelet , platelet concentrate , chemistry , medicine
Background and Objectives Lowering the plasma content in single‐donor platelet (PLT) concentrates well below 30% implies the need to collect platelets at very high concentrations. Trima Accel (TA) is validated for collection below 4000 × 10 3 PLTs/µl. We evaluated its performance at 5000 × 10 3 PLTs/µl. Materials and Methods Twenty blood donors underwent apheresis with TA twice collecting either a hyperconcentrated or a standard single‐donor platelet concentrate with a target platelet concentration of 5000 or 1200 × 10 3 PLTs/µl, respectively. We analysed the collection efficiency, the collection rate and the quality of the collected by‐plasma. Results We collected 20 hyperconcentrated and 20 standard units containing 2·56 ± 0·5 and 3·39 ± 0·4 × 10 11 PLTs at a concentration of 4518 ± 978 and 1374 ± 166 × 10 3 PLTs/µl in 45 ± 8 and 39 ± 6 min resulting in a collection efficiency of 47·5 ± 10·0 and 70·7 ± 7·9% and a collection rate of 5·9 ± 1·4 and 8·8 ± 1·5 × 10 9 PLTs/min, respectively (all results expressed as mean ± standard deviation). The collected by‐plasma showed a very high grade of cell purity and a satisfactory recovery of the clotting factors. Conclusion Although TA is a suitable device for PLT collection at very high concentrations, improvements are desirable to further increase the productivity above its currently validated upper collect concentration limit.