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Comparison of flow cytometry vs. a haematology cell analyser‐based method to guide the optimal time‐point for peripheral blood stem cell apheresis
Author(s) -
Gutensohn K.,
Magens M.,
Krüger W.,
Kröger N.,
Kühnl P.
Publication year - 2006
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.2005.00720.x
Subject(s) - apheresis , flow cytometry , analyser , stem cell , cd34 , haematopoiesis , hematology , progenitor cell , cytometry , medicine , peripheral blood , immunology , blood cell , myeloid , platelet , biology , chemistry , chromatography , genetics
Background and Objectives For timing the onset of apheresis, parameters obtained by flow cytometry and by a haematological cell analyser were compared. Materials and Methods Haematopoietic cell counts ( n = 159) were performed by two different methods; CD34 analyses by flow cytometry, immature myeloid information (IMI) and human progenitor cell counts (HPC) by a haematological cell analyser. Results Comparing the IMI total results with CD34+ analyses ( n = 159) revealed a correlation of r = 0·46 ( P < 0·05). Similar results were obtained for HPC ( r = 0·44; P < 0·05). Conclusion The haematology analyser‐based method does not allow the precise determination of absolute haematopoietic stem cell numbers and is thus not able to replace flow cytometry for the monitoring of peripheral blood stem cell counts.