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Separation of whole blood into plasma and red cells by using a hollow‐fibre filtration system
Author(s) -
Hornsey V. S.,
McColl K.,
Drummond O.,
Prowse C. V.
Publication year - 2005
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.2005.00660.x
Subject(s) - filtration (mathematics) , chromatography , plasma , chemistry , mathematics , physics , statistics , quantum mechanics
Background and Objectives The aim of this study was to assess the separation of whole blood into red cells and plasma by using the Sangofer® device, which is a gravity‐fed, hollow‐fibre system. The components would then be compared with those produced by the use of more elaborate technical equipment. Materials and Methods Ten whole‐blood units were leucoreduced by using a WBF2 filter and immediately separated into red cells and plasma by using the Sangofer® blood‐separation device. Red cells were stored in additive solution and tested on days 1 and 42. The plasma was assayed for levels of various coagulation factors and for markers of both coagulation and complement activation. Results The red‐cell parameters were similar to those obtained when routine centrifugation methods were used. The filter did not cause haemolysis. Levels of plasma factor VIII and factor XI were lower than those seen in routinely produced leucoreduced plasma units but there was no evidence of activation of the coagulation and complement systems. Conclusions The Sangofer® device is simple and straightforward to use and eliminates the need for both centrifugation and automated separation steps during the processing of whole blood into red cells and plasma components. Minor changes are required to make the procedure easier to incorporate into routine use.