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White blood cell fragments in platelet concentrates prepared by the platelet‐rich plasma or buffy‐coat methods
Author(s) -
DijkstraTiekstra M. J.,
Van Der Schoot C. E.,
Pietersz R. N. I.,
Reesink H. W.
Publication year - 2005
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.2005.00634.x
Subject(s) - buffy coat , platelet , platelet rich plasma , blood cell , chemistry , white blood cell , medicine , immunology
Background and Objectives White blood cell (WBC) fragments in platelet concentrates (PCs) may induce allo‐immunization in the recipient. Materials and Methods As the level of WBC fragments can differ between PCs produced using different methods, we compared PCs prepared by using the buffy‐coat method (BC‐PCs) in plasma or platelet additive solution (Composol) and PCs prepared using the platelet‐rich plasma method (PRP‐PCs). Results Post‐filtration results revealed identical levels of WBC, but significantly higher CD62p expression and a significantly lower amount of total DNA, cell‐free DNA and number of WBC fragments (0·6 vs. 4·2 WBC equivalents/µl) in PRP‐PCs than in BC‐PCs in either plasma or Composol. Conclusions The number of WBC fragments is significantly higher in BC‐PCs than in PRP‐PCs.