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In vitro function of platelet concentrates prepared after filtration of whole blood or buffy coat pools
Author(s) -
Turner C. P.,
Sutherland J.,
Wadhwa M.,
Dilger P.,
Cardigan R.
Publication year - 2005
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.2005.00609.x
Subject(s) - buffy coat , platelet , andrology , cd63 , platelet activation , chemistry , whole blood , in vitro , annexin , immunology , biochemistry , medicine , microrna , microvesicles , gene
Background/method Data on the quality of platelet concentrates (PC) produced by the buffy coat method and stored beyond 5 days in plasma are limited. We therefore evaluated the quality of PCs prepared by leucocyte depletion of whole blood (Terumo WBSP, n = 10) or a buffy coat pool (Pall Autostop, n = 10), and stored for 7 days in plasma by assessing platelet parameters and markers of platelet activation. Results In both types of PC, levels of glucose decreased during storage but were not totally depleted (> 11 mM on day 7). In contrast, lactate levels increased on storage and was consistently < 20 mM throughout, with pH maintained at > 6·8 in all units. Hypotonic shock response scores were > 47% in all units at day 7. On day 1, markers of platelet activation were significantly higher in WBSP PC, but by day 7 were similar for percentage CD63+ and CD62P + (40%) with levels of platelet microparticles and annexin V binding two‐fold higher in WBSP. The expression of CD61 did not alter during storage and the percentage of platelets expressing CD42b was > 88% in all units on day 7. RANTES (Regulated on activation, normal, T‐cell expressed and secreted) and TGF β released from platelets by day 7 was < 800 ng/ml and 90 ng/ml, respectively. C3a desarg increased throughout storage in both types of PC, but without a commensurate increase in the terminal complex SC5b‐9 or activation of factor XII. Conclusion Our data indicates that the in vitro characteristics of PCs prepared using these methods is maintained over storage for 7 days in plasma and is not associated with significant deterioration of platelet function. One sentence summary In vitro function of platelet concentrates prepared by either filtration of whole blood, or pooled buffy coats