Premium
A simple spectrophotometric method for the quantification of residual haemoglobin in platelet concentrates
Author(s) -
Cookson P.,
Sutherland J.,
Cardigan R.
Publication year - 2004
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.2004.00566.x
Subject(s) - chromatography , chemistry , coefficient of variation , platelet , residual , accuracy and precision , analytical chemistry (journal) , mathematics , medicine , statistics , algorithm
Background and Objectives High levels of residual haemoglobin (Hb 0·1 g/l) are known to decrease the efficiency of pathogen‐inactivation systems. We evaluated three separate methods to quantify Hb in platelet concentrates (PC). Materials and Methods Nine PC prepared in platelet additive solution (PASIII) (median platelet yield of 283 × 10 9 /unit, range 46–353) were spiked to known Hb concentrations with whole blood and the samples were measured by using each of three methods: the 3,3′,5,5′‐tetramethylbenzidine (TMB) oxidation method (Sigma Diagnostics, 527‐A); the Harboe spectrophotometric method; and the HemoCue plasma low‐Hb photometer (PLHP). Results The TMB and Harboe methods showed linear results compared to expected Hb ( r 2 ≥ 0·981, P < 0·001) over the range tested (0·09–0·28 g/l) when the samples were haemolysed. The TMB method underestimated by an average of 6%, at and around 0·1 g/l Hb, compared to a 4% overestimation by the Harboe method and a threefold overestimation by the PLHP. The Harboe intra‐assay coefficient of variation was ≤ 1·85% across all concentrations, which contrasted with 30% at and around 0·1 g/l for the TMB method. Conclusions The Harboe spectrophotometric method is convenient, safe, accurate and reproducible, and outperforms the TMB and PLHP methods for quantification of residual Hb in PC.