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Platelet characteristic antigens of CD34 + cells in cryopreserved cord blood: a study of platelet‐derived microparticles in transplant processing
Author(s) -
Liu B.,
Chen J. S.,
Cao M.,
Gu S. L.,
Liao C.,
Li D. Z.,
Zhong H. Z.
Publication year - 2004
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.2004.00538.x
Subject(s) - platelet , cord blood , cd34 , cryopreservation , andrology , chemistry , transplantation , thrombin , immunology , antigen , microbiology and biotechnology , stem cell , biology , medicine , surgery , embryo
Background and Objectives In previous studies, we found that platelet microparticles (PMPs) bind to cord blood (CB) CD34 + cells and transfer adhesion molecules to them, which enhances their engraftment. Before applying this phenomenon in actual transplants, we investigated the effect of PMPs on cryopreserved CD34 + cells in CB. Materials and Methods We cryopreserved 18 CB units, then evaluated the binding of PMPs to CD34 + cells after thawing, by varying the expression of platelet characteristic antigens (CD41a, CD61, CD62P and CXCR4) on these cells. Adherence of the CD34 + cells, coated with freeze/thaw‐induced PMPs, to endothelium and fibronectin was also studied, as were the effects of thrombin‐induced PMPs from both fresh and preserved CB platelets. Results PMPs induced by freezing and thawing adhered less well to CD34 + cells than did those from fresh CB, and cells coated with these PMPs had poor adherence. However, thrombin‐induced PMPs from both fresh and preserved CB platelets bound equally well to cryopreserved CD34 + cells and improved their adhesion properties. Conclusions PMPs could be a useful tool for enhancing engraftment after CB transplantation.