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Inheritance of Abnormal Glycophorin C of the Gerbich and Yussef Type in a French Family
Author(s) -
Loirat M.J.,
PineauVincent F.,
Schiffer C.,
Muller J. Y.,
Blanchard D.
Publication year - 1996
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1996.tb01299.x
Subject(s) - glycophorin , monoclonal antibody , microbiology and biotechnology , allele , antibody , biology , antigen , red blood cell , trypsin , monoclonal , genetics , gene , immunology , biochemistry , enzyme
The discovery of a natural Gerbich antigen (anti‐Ge2) in the serum of a propositus prompted us to study his red blood cells (RBCs) by using monoclonal antibodies (mAbs) directed against glycophorin (GP) C and GPD. An mAb directed against the Ge4 antigen (mAb NaM10‐7G11) agglutinated both untreated and trypsin‐treated cells, demonstrating the expression of a trypsin‐resistant GPC (namely, GPC of the Gerbich type: GPC Ge ). Surprisingly, an anti‐Ge3 antibody (mAb NaM19‐3C4) agglutinated untreated cells, showing that they also express the Ge3 antigen that may be carried by normal GPC and CPD or by the abnormal GPC of the Yussef (Yus) type (GPC Yus ). Immunoblotting analysis performed with an mAb directed against the C‐terminal portion of GPC showed that the propositus' RBCs do not contain normal GPC and GPD but both GPC Ge and GPC Yus . Analysis of RBCs from the family demonstrated that, like the propositus, 2 of the 3 sisters had inherited both the GYPC Ge and the GYPC Yus alleles from the parents, who carried either the GYPC Ge or the GYPC Yus allele. The third sister had inherited the normal GYPC alleles from her parents, whereas the child of the propositus had inherited the GYPC Ge allele. Interestingly, natural anti‐Ge2 antibodies were identified in the serum of 2 of the 3 Ge‐negative individuals.