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Genotype Frequencies of the Human Platelet Antigen, Ca/Tu, in Japanese, Determined by a PCR‐RFLP Method
Author(s) -
Tanaka Shigenori,
Taniue Atsuko,
Nagao Nobuo,
Tomita Tadao,
Ohnoki Shiro,
Shibata Hirotoshi,
Okubo Yasuto,
Yamaguchi Hideo,
Shibata Yoichi
Publication year - 1996
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1996.tb00995.x
Subject(s) - platelet , genotype , microbiology and biotechnology , antigen , restriction fragment length polymorphism , complementary dna , amino acid , population , biology , chemistry , gene , biochemistry , immunology , medicine , environmental health
Recently, the polymorphism of a new human platelet antigen, Ca/Tu, was shown to be derived from a G‐A nucleotide substitution at base 1564 of GPIIIa cDNA, which leads to a single amino acid difference, Arg/Gln at amino acid 489 of GPIIIa. We developed a PCR‐RFLP method to determine the genotypes of Ca/Tu and their frequencies in a Japanese population. Fifteen Ca/Tu a donors comprising 1 Ca/Tu(a/a) homozygous donor and 14 Ca/Tu(a/b) heterozygous donors were found among the 314 random donors analyzed. The frequencies of Ca/Tu genes were 0.025 (Ca/Tu a ) and 0.975 (Ca/Tu b ). The present study showed that the frequency of Ca/Tu a individuals in the Japanese (15/314) was approximately 7‐fold higher than in the Finnish population (1/150) previously reported by Kekomäki et al. Therefore, attention must be given to the involvement of the Ca/Tu alloantigen in neonatal alloimmune thrombocytopenia and the refractoriness of platelet transfusion.