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Investigation of the Biochemical Relationship between the Blood Group Antigens Xg a and CD99 (12E7 Antigen) on Red Cells
Author(s) -
Petty Alison C.,
Tippett Patricia
Publication year - 1995
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1995.tb02600.x
Subject(s) - immunoprecipitation , antigen , cd99 , microbiology and biotechnology , red blood cell , biology , antibody , cell membrane , membrane protein , band 3 , chemistry , membrane , biochemistry , immunohistochemistry , immunology , vimentin
The biochemical relationship between the red cell antigens Xg a and the MIC2 gene product, CD99 – previously designated the 12E7 antigen – has been examined by immunoblotting and immunoprecipitation analyses of the protein molecules bearing these antigens. Immunoblotting of membrane components and Xg a ‐immunoprecipitates with anti‐Xg a has shown that Xg a antigen is carried on a broad band of apparent molecular weight (M r ) 24,500–29,500, which consists of a darkly stained component at M r 24,500 and a more diffusely stained component at approximately M r 26,500–29,500. Immunoblotting of membrane components and 12E7‐immunoprecipitates with 12E7, and RFB‐1 and NaM123 which also recognise CD99, distinguished two bands of M r 30,000 and 32,000. A non‐radioactive immunoprecipitation technique was employed, which uses chemiluminescence detection of biotin‐labelled red cell proteins. The protein of M r 32,000, which carries CD99, was identified by this method and the red cell quantitative polymorphism of CD99 was demonstrated. When the Xg a protein was precipitated from biotin‐labelled red cells, a protein of M r 32,000 was coprecipitated. This suggests that the proteins carrying the Xg a antigen and CD99 are associated in the membrane.