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A Comparative Study of Platelets Stored in Polyvinyl Chloride Containers Plasticised with Butyryl Trihexyl Citrate or Triethylhexyl Trimellitate
Author(s) -
Turner V. S.,
Mitchell S. G.,
Kang S. K.,
Hawker R.J.
Publication year - 1995
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1995.tb02593.x
Subject(s) - polyvinyl chloride , blood preservation , platelet , chemistry , waste management , nuclear chemistry , organic chemistry , medicine , andrology , engineering
Platelet concentrates (PCs), stored for 5 days in PL 2209, a new polyvinyl chloride (PVC) storage container plasticised with butyryl trihexyl citrate, were compared with those stored in PL 1240, a PVC platelet container plasticised with triethylhexyl trimellitate. In part 1 of the study, pooled platelet‐rich plasma (PRP) was aliquoted into each type of pack and pH, pCO 2 , pO 2 , hypotonic shock response, aggregation responses, lactate, glucose and ATP concentrations, and lactate dehydrogenase and β‐thromboglobulin release were compared at days 1, 3 and 5. In part 2, 12 volunteers gave a unit of blood on two separate occasions and PCs produced by the PRP method were stored in PL 2209 or PL 1240 for 5 days before autologous reinfusion of a 111 In‐labelled sample. In vitro results demonstrated that PL 2209 was more gas permeable than PL 1240. In part 2 of the study, at day 5, pCO 2 was 3.13±0.62 versus 5.14±0.69 (p<0.001), whilst pO 2 was not significantly different for PL 2209 versus PL 1240, respectively. pH was better maintained in PL 2209 than in PL 1240 (7.38±0.13 vs. 7.24±0.10, respectively, p<0.01) after storage for 5 days. These results were confirmed by those from part 1. In vivo data were similar for PC stored in the two plastics with a multiple‐hit recovey of 40.9±12.1% for PL 2209 and 37.4±11.3% for PL 1240, and a multiple‐hit survival of 4.89±1.20 days and 5.28±2.06 days for PL 2209 and PL 1240, respectively. γ‐Camera imaging of volunteers showed similar biodistribution of radiolabeled platelets stored in each container. These results demonstrate that PL 2209 is a suitable container for storage of PCs for 5 days.