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Simultaneous DNA Typing of Human Platelet Antigens 2, 3 and 4 by an Allele‐Specific PCR Method
Author(s) -
Tanaka Shigenori,
Taniue Atsuko,
Nagao Nobuo,
Ohnoki Shiro,
Shibata Hirotoshi,
Okubo Yasuto,
Yamaguchi Hideo
Publication year - 1995
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1995.tb02577.x
Subject(s) - genotyping , polymerase chain reaction , genotype , typing , allele , restriction enzyme , restriction fragment length polymorphism , microbiology and biotechnology , antigen , hemagglutination , platelet , allele frequency , biology , dna , immunology , genetics , gene
We developed an allele‐specific polymerase chain reaction (ASPCR) method using originally designed primers to determine the genotype of the human platelet antigens (HPAs) 2, 3 and 4 in parallel. The results were compared with those obtained by PCR restriction fragment length polymorphism and the mixed passive hemagglutination test. Seventy‐three individuals were tested and the ASPCR results were in good agreement with those determined by the other two methods. This method enables the genotyping of HPA‐2, ‐3 and ‐4 in parallel without the use of platelets, platelet‐specific alloantibodies or restriction enzymes.