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Growth Factor Release during Preparation and Storage of Platelet Concentrates
Author(s) -
Ledent E.,
Wasteson Å.,
Berlin G.
Publication year - 1995
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1995.tb02573.x
Subject(s) - platelet , buffy coat , chemistry , growth factor , platelet derived growth factor receptor , platelet derived growth factor , lactate dehydrogenase , andrology , beta thromboglobulin , platelet rich plasma , platelet factor 4 , biochemistry , medicine , immunology , biology , enzyme , receptor
The platelet content of platelet‐derived growth factor (PDGF), a mitogen stored in the alpha‐granules, was studied during preparation and storage of platelet concentrates (PC) and compared to the growth‐promoting activity of platelets, β‐thromboglobulin (β‐TG) and lactate dehydrogenase (LD). We compared PC prepared from platelet‐rich plasma (PRP‐PC; n= 10) and from buffy coat. Two different pre‐preparation storage periods of the buffy coat were used: 4h (BC‐PC:4h; n = 10) and 24 h (BC‐PC: 24h; n = 5). The platelet content of PDGF and β‐TG was measured by a RIA technique and the growth‐promoting activity by incorporation of 3 H‐thymidine in stimulated fibroblasts. The platelet content of PDGF, β‐TG and the growth‐promoting activity of the platelets decreased in a similar way during preparation and storage of PRP‐PC (31 ±2, 35±2 and 33±7%, respectively, at day 5 of storage; mean ± SEM). The release of LD was minor (3.9 ±0.5% at day 5). At day 1 of storage the platelet content of PDGF was significantly better preserved in BC‐PC:4h than in BD‐PC:24h (88±2 and 81 ±3%, respectively; p = 0.03). Comparing BC‐PC:4h and PRP‐PC we found a significantly better preservation of PDGF in BC‐PC:4h until day 3 of storage (80±2 and 75±1%, respectively at day 3; p = 0.046). In conclusion the preparation of PC according to the PRP method initially induces a higher loss of PDGF, and hence of the growth‐promoting activity, than the BC method.