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Human Cytomegalovirus DNA Is Not Detectable with Nested Double Polymerase Chain Reaction in Healthy Blood Donors
Author(s) -
Urushibara Noriko,
Kwon KilWon,
Takahashi Tsuneo A.,
Sekiguchi Sadayoshi
Publication year - 1995
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1995.tb02537.x
Subject(s) - nested polymerase chain reaction , polymerase chain reaction , cytomegalovirus , ethidium bromide , dna , biology , virology , microbiology and biotechnology , human cytomegalovirus , betaherpesvirinae , real time polymerase chain reaction , primer (cosmetics) , herpesviridae , gene , viral disease , virus , chemistry , genetics , organic chemistry
The PCR method was introduced to detect cytomegalovirus (CMV) DNA from 189 peripheral blood samples of volunteer donors. We adopted the nested double PCR method with primers specific for immediate early gene 1 followed by electrophoresis and ethidium bromide staining. This nested double PCR method was sensitive enough to detect approximately a single copy of CMV DNA. However, we failed to obtain positive amplification of CMV DNA from any of these donor samples. In contrast, CMV DNA could be detected in all 3 tested immunocompromised patients who had undergone bone marrow transplantation. These results support our previous report that the frequency of CMV DNA is of an order lower than 1 copy/10 5 leucocytes in the peripheral blood of healthy seropositive individuals.