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Effect of Filtration on Subsequently Stored Platelet Concentrates
Author(s) -
JoustraDijkhuis A.M.,
Boomgaard M.N.,
Pietersz R.N.I.,
Steneker I.,
Korte D.,
Loos J.A.,
Reesink H.W.
Publication year - 1994
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1994.tb05032.x
Subject(s) - filtration (mathematics) , platelet , chemistry , buffy coat , chromatography , bicarbonate , andrology , biology , surgery , medicine , immunology , mathematics , statistics , organic chemistry
The effect of filtration on the quality of platelet concentrates (PC) during storage was investigated. Two leukocyte depletion filters (Pall PL50HF and Sepacell PL‐10A) were applied to filter PC made from a pool of 4 buffy coats. For each experiment 3 PC were pooled and divided into 3 identical PC to eliminate differences between the PC. Two PC were filtered, and the third PC served as an unfiltered control. A total of 12 experiments was performed. Before filtration, volumes of the PC were 263±11.7 ml (mean±SD). Platelet and leukocyte counts per PC were 241±25.9 times 10 9 and 7.2±1.8 times 10 6 , respectively. After filtration leukocyte counts did not exceed 5 times 10 4 in any of the PC. In the PC filtered with the Pall PL50HF the mean platelet loss was approximately 14% and with the Sepacell PL‐10A, 17%. During a 9‐day storage period the pH, PO 2 , PCO 2 , bicarbonate, lactate and glucose concentration and LDH release as well as the morphology, examined by the swirling effect and microscopically, were not significantly different in filtered and unfiltered units. Filtration through the 2 investigated leukocyte depletion filters for PC did not adversely affect in vitro viability of the platelets during storage.