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Comparative Immunochemical Analysis of Wr a and Wr b Red Cell Antigens
Author(s) -
Ring S. M.,
Tippett P.,
Swallow D. M.
Publication year - 1994
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1994.tb01665.x
Subject(s) - glycophorin , immunoprecipitation , antigen , monoclonal antibody , microbiology and biotechnology , red blood cell , biology , band 3 , antibody , monoclonal , erythrocyte membrane , immunology , membrane , biochemistry
This paper describes immunoblotting and immunoprecipitation studies using monoclonal anti‐Wr a and anti‐Wr b antibodies to investigate the nature of the lowincidence blood group antigen, Wr a and its high‐incidence allelic antigen Wr b . No membrane components were identified by the immunoblotting experiments. Immunoprecipitation studies confirmed that the Wr b antigen involves both glycophorin A and band 3. The monoclonal anti‐Wr a BGU1‐WR, failed to immunoprecipitate these or any other red cell membrane component. The significance of these findings is discussed.