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Anti‐j: Human Cold Agglutinins Recognizing Linear (i) and Branched (I) Type 2 Chains
Author(s) -
Roelcke Dieter,
Kreft Heidi,
Hack Hildegard,
Stevenson Freda K.
Publication year - 1994
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1994.tb01663.x
Subject(s) - idiotype , antibody , cold agglutinin , antigen , chemistry , protease , sequence (biology) , microbiology and biotechnology , biology , immunology , biochemistry , enzyme , monoclonal antibody
Abstract Two IgMΛ cold agglutinins (CAs) reacted with protease‐ and sialidase‐resistant antigens expressed in equal strength on human adult (I), newborn (i), i adult, rabbit (I) and rhesus monkey (i) erythrocytes. The antibodies were inhibited by the linear type 2 sequence lacto‐N‐neotetraose and the branched type 2 sequence lacto‐N‐neohexaose. Endo‐β‐galactosidase treatment of red cells, which splits type 2 chains from the surface, abolished CA reactivity. The CAs expressed the idiotype recognized by the anti‐idiotype 9G4 specific for anti‐I and anti‐i CAs. The data suggest that the two CAs recognize linear (i) as well as branched (I) type 2 chains. It is proposed to term these CAs anti‐j.